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At BPI Tech, we are committed to revolutionize the art and science of biopolymer isolations and purifications, especially nucleic acids and proteins from a variety of biological sources. Our technology works around the type of biological samples processed, rather than blindly adopting a particular protocol to any and all biological samples. We place great emphasize on understanding the chemistry of the sample type, whether it is soil, plant, mammalian tissues or microbes, and our isolation protocols work around the chemistry of these types of samples. To keep up with the advances in instrumentations, we constantly strive to improve the sensitivity of our isolation protocols, placing importance on both, quantitative isolation and qualitative isolation.  Our isolation methods include direct precipitation of nucleic acids from homogenized samples, use of silica membranes and silica-coated magnetic beads.

Unique features of our kits:
Store samples in Lysis Buffer for up to 7 days at room temperature
One minute precipitation of nucleic acid
Our patent-pending pellet washing method to remove contaminants
Hand-held kits for field use
Nucleic acid storage cards for both liquid and solid samples

DNA Isolation

Our DNA extraction kits are the quickest in the market, from homogenization to elution within 15 minutes, for any of your sample type.  You have the option of hand homogenization or mechanical homogenization for plant samples and mammalian tissues.  Irrespective of the sample type, our protocols consist of five reagents: Lysis Buffer, Bind Buffer, Wash Buffer I & II, and Elution Buffer.  Wash Buffer II is 70% ethanol and Elution Buffer is sterile water.  Homogenized samples are centrifuged and the supernatant is bound to silica matrices in the presence of Bind Buffer.

The core chemistry of our protocol is a patent-pending pellet purification step where total nucleic acid is instantaneously precipitated to enhance both quality and quantity.  For complex samples high in polyphenols, we have incorporated a one-minute precipitation step to pellet nucleic acids and the contaminants followed by this patent-pending pellet wash that neutralizes organic contaminants. This further enable the end-user to customize the protocol as per their requirements.  Our field-isolation kits enable researchers to process their samples immediately after collection, circumventing the need for storage and avoiding possible changes in the sample profiles.

 

RNA Isolation

RNA isolation is the primary step in gene expression studies.  mRNA is usually the target molecule in RNA isolation while other species of RNA provide valuable information in the study of living organisms.
RNA is highly susceptible to degradation by RNases during extraction processes and therefore, it is more difficult to recover RNA than DNA. Most strategies for mRNA isolation involves as a first step, isolation of total RNA and a secondary step for isolating or enriching for mRNA.  Apart from RNases, co-extracted DNA and based on the sample type, other phenolic inhibitors could render RNA unusable for such fundamental procedures such as reverse transcription and transcriptomic studies.
Our protocol aims to isolate total RNA from biological samples, from blood to soil samples using our proprietary non-chaotrophic Lysis Buffer to inactivate nucleases and to nuetralize phenolic inhibitors.  Our protocol ensures the isolation of all species of RNA molecules.

Lab Accessories

We offer lab accessories tailored to every step in nucleic acid isolation from homogenizing beads up to Taq enzymes.
We have a wide range of homogenizing beads to cover all your homogenizing needs for any sample type.  Our collection of spin columns include 3 different types of silica membranes and in 6 different volumes.
Our reagents include lysis buffers for DNA isolation and RNA isolation along with corresponding bind buffers.  We also offer clean-up reagent set to improve nucleic acid quality.
The other accessories include nucleic acid storage cards, 96 well plates, magnetic beads and filtration columns of various pore sizes. Our Taq enzyme collection includes 6 different types for all your amplification needs.

At BPI Tech, we are committed to revolutionize the art and science of biopolymer isolations and purifications, especially nucleic acids and proteins from a variety of biological sources. Our technology works around the type of biological samples processed, rather than blindly adopting a particular protocol to any and all biological samples. We place great emphasize on understanding the chemistry of the sample type, whether it is soil, plant, mammalian tissues or microbes, and our isolation protocols work around the chemistry of these types of samples. To keep up with the advances in instrumentations, we constantly strive to improve the sensitivity of our isolation protocols, placing importance on both, quantitative isolation and qualitative isolation.  Our isolation methods include direct precipitation of nucleic acids from homogenized samples, use of silica membranes and silica-coated magnetic beads.

Unique features of our kits:
Store samples in Lysis Buffer for up to 7 days at room temperature
One minute precipitation of nucleic acid
Our patent-pending pellet washing method to remove contaminants
Hand-held kits for field use
Nucleic acid storage cards for both liquid and solid samples