DNA Isolation Buffers

DNA Lysis Buffer
A powerful combination of organic and inorganic salts with a unique  detergent 

Effective lysis of prokaryotic and eukaryotic cells
Powerful protein denaturant
Neutralizes polyphenols and polysaccharides
Binding nucleic acids to silica membranes and silica-coated magnetic beads

 

Catalog # Size Price
DLB-01-50 50 ml $ 22.00
DLB-01-100 100 ml $ 40.00

DNA Bind Buffer
A unique combination of organic salts with a proprietary solvent

Proprietary mix of solvents and salts
Binds nucleic acids to silica membrane and silica-coated nucleic acids
Add two volumes of DNA Bind Buffer to one volume of nucleic acid solution
Selective binding of nucleic acids in the presence of polyphenols and polysaccharides

 

Catalog # Size Price
DBB-01-50 50 ml $ 15.00
DBB-01-100 100 ml $ 30.00

Use our DNA Isolation Buffers to isolate DNA from any sample type

Isolate DNA from whole blood by directly adding  DNA Lysis Buffer to blood or resuspend  white blood cells in DNA Lysis Buffer

  • For whole blood:
  • Add 400 µl of DNA Lysis Buffer for every 200 µl of blood
  • Invert by Mixing and incubate for 5 minutes at room temperature
  • Add an equal volume of our DNA Bind Buffer or 100% ethanol
  • Add an equal volume of our DNA Bind Buffer or 100% ethanol
  • Process through silica membrane spin columns or silica-coated magnetic beads

For White blood cell pellet:

  • Resuspend the pellet in DNA Lysis Buffer at the same proportion as above and follow the above protocol

Saliva, Sputum and Buccal Cells:

  • Pellet microbial cells
  • Add 800 µl of DNA Lysis Buffer and resuspend the cell pellet
  • Homogenize in a bead tube
  • Centrifuge and remove supernatant
  • Add an equal volume of DNA Bind Buffer or 100% Ethanol
  • Bind either to spin columns or silica coated magnetic beads
  • Use up to 15 mg for fresh samples and for dry samples, the amount will vary
  • Use up to 800 µl of DNA Lysis Buffer and homogenize the samples in a bead tube
  • Centrifuge the supernatant for 5 minutes
  • Add 800 µl of DNA Bind Buffer and process through either a silica membrane spin column or silica-coated magnetic beads
  • For up to 30 mg, use 800 µl of DNA Lysis Buffer
  • The amount of crushed seed material will vary depending on the polysaccharide content and lipid content of seeds (You can use our hand-held homogenization tubes, catalog # GS-01s-50 for this application)
  • Centrifuge and remove the supernatant to a pre filter column (FC-01-100) and centrifuge for 5 minutes
  • Add 800 µl of DNA Bind Buffer and process through a silica membrane spin column or silica-coated magnetic beads
  • Use 800 µl of DNA Lysis Buffer for up to 30 mg of plant materials
  • Homogenize the samples in a bead tube.  You can use our hand-held homogenization system (catalog # GS-01s-50)
  • Remove the supernatant and centrifuge for 2 minutes
  • To the flow-through, add 800 µl of DNA Lysis Buffer and process through a silica membrane spin column or silica-coated magnetic beads