Fecal RNA Isolation

Our Kit

A Mini kit to process up to 50 mg of fecal samples
Successful and quality DNA from both fresh and dried samples
Bead tubes with 3 different bead types in 5 different sizes
Direct binding to silica membranes or precipitation
Patent pending pellet purification
No Phenol:Chloroform purification
No Anion Exchange column purification
Reduced hands-on time

Fecal is a valuable sample in diagnostic studies.  The composition of intestinal microbiota is now considered to have a direct bearing on the health of an individual and structural alterations are considered to result in several diseases.  It is possible to study the functional composition of gut microbiota through excreted feces.

Apart from its microflora, it is also a storehouse of several genetic markers.  Gene expression analyses of specific genes are being investigated as a potential tool for early detection of several diseases and mutations.
Fecal samples are also used in the early detection and diagnosis of several viral diseases in both, humans and animals.
Numerous studies have been published using feces as a non-invasive diagnostic tool to study migratory birds and wild animals.

Lyse’N’Pellet
Direct precipitation and purification of RNA without the aid of solid phase purification.

Protocol Overview

Homogenization
                  ⇓
Centrifugation
                  ⇓
Pre filtration
                  ⇓
1 minute Precipitation
                  ⇓
Pellet Washing
                  ⇓
Resuspension

Mini Prep            [maxbutton id=”3″]

  Catalog Number Size Price
FLRMN-01-10 10 Preps $ 45.00
FLRMN-01-50 50 Preps $ 150.00
FLRMN-01-100 100 Preps $ 275.00

Lyse’N’Trap
Purification of RNA with the aid of silica membrane spin columns.

Protocol Overview

Homogenization
                 ⇓
Centrifugation
                ⇓
Pre filtration
                ⇓
Binding
                ⇓
Washing
                ⇓
Elution

Mini Prep                    [maxbutton id=”3″]

  Catalog Number Size Price
FLRMN-02-10 10 Preps $ 45.00
FLRMN-02-50 50 Preps $ 200.00
FLRMN-02-100 100 Preps $ 350.00