FS Taq Polymerase

FS Taq  Polymerase
It is the latest generation of Taq-based polymerases that can shorten the amplification time by a third. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does can be used in a variety of PCR. The FS PCR Buffer, designed for FS Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FS Taq DNA polymerase can be as much as 3 kb/min.

Routine PCR
DNA labeling
PCR sequencing
Generation of PCR products for TA cloning

Fast elongation rate; up to 3 kb/min
3 times faster than Taq DNA polymerase
Thermostable -half-life over 40 min. at 95˚C 
It has 5′ to 3′ polymerase activity
Lacks 3′ to 5′ exonuclease activity
Generates 3′ -dA overhangs PCR products

Storage Buffer
20mM Tris HCl (pH 8.0)
100 mM KCl
1 mM DTT
0.5% NP-40
0.5% Tween 20
0.2 mg/ml BSA
50% (v/v) glycerol

10X FS PCR Buffer with Mg2+
200 mM Tris-HCl (pH 8.8)
100 mM KCl
1% Triton X-100

16 mM MgSO4
100 mM (NH4)2SO4

Catalog # Size Price
TP-31-01 500 U $ 67.50
TP-31-02 1000 U $ 121.50

FS Taq Mix
FS Mix (2X) is a pre-mixed, read-to-use solution containing FS Taq DNA Polymerase, dNTPs, and all other PCR components, except DNA template and primers. FS Taq Mix contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. FS Mix (2X) can be used with conventional PCR machines.

High throughput PCR
Long and Complex template PCR
Routine PCR with high reproducibility
Generation of PCR products for TA cloning

Composition of 2X FS Mix
0.3U/μl FS Taq DNA Polymerase
2X FS PCR Buffer
0.4 mM dNTPs

3.2 mM MgSO4
0.02% Bromophenol blue

Reduced hands-on time
Higher efficiency
Higher sensitivity

Catalog # Size Price
TP-32-01 1 ml $ 60.00
TP-32-02 1 x 5 ml $ 270.00

Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTPs inot an acid-insoluble form in 30 minutes at 70˚C using hering sperm DNA as substrate.

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