HS Taq Polymerase

HS Taq Polymerase
Contributes to highly specific incorporation of nucleotides
Source                                    Thermus aquaticus
Molecular Weight             94 kDa
Elongation Velocity          0.9 –  1.2 kb/min (70-75°C)
Amplification Capacity   Up to 5 kb (simple template)
Polymerase activity          5′ to 3′ polymerase activity
Exonuclease activity         Lacks 3′ – 5′ exonuclease activity that results in a 3′
dA overhangs 

Applications
PCR amplification of DNA fragments as long as 5 kb 
DNA Labelling
DNA sequencing

Storage Buffer
20mM Tris HCl (pH 8.0)
100 mM KCl
3 mM MgCl2

1 mM DTT
0.1% NP-40
0.1% Tween 20
0.2 mg/ml BSA
50% (v/v) glycerol

10X PCR Buffer with Mg2+
200 mM Tris-HCl (pH 8.8)
100 mM KCl
1% Triton X-100

16 mM MgSO4

Catalog # Size Price
TP-29-01 500 U $ 65.00
TP-29-02 1000 U $ 117.00

HS Taq  Mix
HS 2X Taq Mix is a premixed, ready-to-use solution containing HS Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, primers and template DNA are added directly to the aliquots. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. It also contributes to higher specificity by optimizing the system, reducing primer-dimer rate.

Applications
High throughput PCR
High specificity PCR
Routine PCR with high reproducibility
Generation of PCR products for TA cloning

Composition of 2X HS Mix
0.3U/μl HS Taq DNA Polymerase
2X HS PCR Buffer
0.4 mM dNTPs

3.2 mM MgSO4
0.02% Bromophenol blue

Features
Convenience
Reduced hands-on time
Higher efficiency
Reproducibility
Higher sensitivity

Catalog # Size Price
TP-30-01 1 ml $ 53.00
TP-30-02 1 x 5 ml $ 238.00

Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTPs inot an acid-insoluble form in 30 minutes at 70˚C using hering sperm DNA as substrate.

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