Long Taq Polymerase

Long Taq Polymerase
Long Taq DNA Polymerase, a combination of two thermostable DNA polymerase, Taq and Pfu, is a special formulation designed for amplifying large fragments. This specially formulated Long Taq was shown to amplify long templates from ƛ phage genome up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich templates. Long Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long Taq in your PCR reactions results in 3′ -dA overhangs PCR products, which can be used in TA clones.

Applications
PCR amplification of complex templates
DNA sequencing
PCR for cloning

Storage Buffer
20mM Tris HCl (pH 8.0)
100 mM KCl
3 mM MgCl2

1 mM DTT
0.1% NP-40
0.1% Tween 20
0.2 mg/ml BSA
50% (v/v) glycerol

10X PCR Buffer with Mg2+
500 mM Tris-HCl (pH 8.8)
160 mM (NH4)2SO4
25 mM MgCl2
1% Triton X-100

Catalog # Size Price
TP-27-01 500 U $ 190.00
TP-27-02 1000 U $ 342.00

Long Taq Polymerase Mix
2X Long Taq Mix is a pre-mixed, ready-to-use solution containing Long Taq DNA Polymerase, dNTPs, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, primers and template DNA are added directly to the aliquots. Long Taq Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by add enhancer.

Applications
PCR for long templates up to 40 kb
High throughput PCR for templates

Composition of 2X Long Taq Mix
0.25U/μl Long Taq Plus DNA Polymerase
2X Long PCR Buffer
0.4 mM dNTPs

3.2 mM MgCl2
0.02% Bromophenol blue

Features
Convenience
Reduced hands-on time
Higher efficiency
Consistent reproducibility
Longer templates
GC rich or repetitive templates

Catalog # Size Price
TP-28-01 1 ml $ 80.00
TP-28-02 1 x 5 ml $ 360.00

Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTPs inot an acid-insoluble form in 30 minutes at 70˚C using hering sperm DNA as substrate.

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