Taq Polymerase

Taq Polymerase
Source                                    Thermus aquaticus
Molecular Weight             94 kDa
Elongation Velocity          0.9 –  1.2 kb/min (70-75°C)
Amplification Capacity   Up to 5 kb (simple template)
Polymerase activity          5′ to 3′ polymerase activity
Exonuclease activity         Lacks 3′ – 5′ exonuclease activity that results in a 3′ dA overhangs 

Applications
High throughput PCR
Routine PCR with high reproducibility
Generation of PCR products for TA cloning

Storage Buffer
20mM Tris HCl (pH 8.0)
100 mM KCl
3.2 mM MgCl2
1 mM DTT
0.1% Triton X-100
0.1% Tween 20
0.2 mg/ml BSA
50% (v/v) glycerol

10X PCR Buffer with Mg2+
100 mM Tris-HCl (pH 8.8)
500 mM KCl
1% Triton X-100
16 mM MgCl2

Catalog # Size Price
TP-21-01 500 U $ 35.00
TP-21-02 1000 U $ 63.00

Taq Mix

50% (v/v)2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.

Applications

High throughput PCR
Routine PCR with high reprducibility
Generation of PCR products for TA cloning

Composition of 2X Taq Mix
0.25U/μl Taq DNA Polymerase
2x PCR Buffer
0.4 mM dNTPs
3.2 mM MgCl2
0.025 Bromophenol blue

Features
Convenience
Reduced hands-on time
Higher efficiency
Consistent Reproducibility

Catalog # Size Price
TP-22-01 1 ml $ 45.00
TP-22-02 1 x  5 ml $ 190.00

Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTPs inot an acid-insoluble form in 30 minutes at 70˚C using hering sperm DNA as substrate.

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