RNA Isolation Buffers

RNA Lysis Buffer
A powerful combination of organic and inorganic salts with a unique  detergent 

Effective lysis of prokaryotic and eukaryotic cells
Inactivates ribonucleases
Powerful deproteinization, making protein precipitation unnecessary
Neutralizes polyphenols and polysaccharides
Binding nucleic acids to silica membranes and silica-coated magnetic beads

 

Catalog # Size Price
RLB-01-50 50 ml $ 25.00
RLB-01-100 100 ml $ 45.00

RNA Bind Buffer
A unique combination of organic salts with a proprietary solvent

Proprietary mix of solvents and salts
Could be used in the place of 100% ethanol for binding to silica membranes and silica-coated magnetic beads
Add one volumes of RNA Bind Buffer to one volume RNA solution
Selective binding of nucleic acids in the presence of polyphenols and polysaccharides

 

Catalog # Size Price
RBB-01-50 50 ml $ 17.00
RBB-01-100 100 ml $ 30.00

RNasin 

Inhibition of RNA degradation in the following procedures:
cDNA syntehsis
in vitro transcription
in vitro translation
Isolation of mammalian cell fractions that contains mRNA-protein complexes
Separation and identification of specific ribonulease activities
Studies of tumor suppression

 

Catalog # Size Price
RNS-01-50 500 units $ 25.00
RNS-01-100 1000 units $ 50.00

Use our RNA Isolation Buffers to isolate RNA from any sample type

Isolate RNA by resuspending white blood cell pellets in  RNA Lysis Buffer 

Saliva, Sputum and Buccal Cells:

  • Pellet microbial cells
  • Add 800 µl of RNA Lysis Buffer and resuspend the cell pellet
  • Homogenize in a bead tube
  • Centrifuge and remove supernatant
  • Add an equal volume of RNA Bind Buffer or 100% Ethanol
  • Bind either to spin columns or silica coated magnetic beads
  • Use up to 15 mg for fresh samples and for dry samples, the amount will vary
  • Use up to 800 µl of RNA Lysis Buffer and homogenize the samples in a bead tube
  • Centrifuge the supernatant for 5 minutes
  • Add 800 µl of RNA Bind Buffer and process through either a silica membrane spin column or silica-coated magnetic beads
  • For up to 30 mg, use 800 µl of RNA Lysis Buffer
  • The amount of crushed seed material will vary depending on the polysaccharide content and lipid content of seeds (You can use our hand-held homogenization tubes, catalog # GS-01s-50 for this application)
  • Centrifuge and remove the supernatant to a pre filter column (FC-01-100) and centrifuge for 5 minutes
  • Add 800 µl of RNA Bind Buffer and process through a silica membrane spin column or silica-coated magnetic beads
  • Use 800 µl of RNA Lysis Buffer for up to 30 mg of plant materials
  • Homogenize the samples in a bead tube.  You can use our hand-held homogenization system (catalog # GS-01s-50)
  • Remove the supernatant to a pre filter column and centrifuge for 2 minutes
  • To the flow-through, add 800 µl of DNA Lysis Buffer and process through a silica membrane spin column or silica-coated magnetic beads