Soil DNA Isolation

BPI Tech has simplified Soil nucleic acid isolation without compromising quality and quantity. It is the quickest protocol in the industry. Soil Matrix is complex, heterogeneous and variable as a result of its structural organization.  It appears in aggregates of different sizes and stability, which results in mosaic of micro environments for microorganisms to either thrive or survive, depending on nutrient availability and environmental factors.  The exceptional microbial diversity and intrinsic variations found in structural organization of soil matrix will influence the amount of soil samples used in DNA extractions.  Humic acids and other phenolic compounds are major contaminants of soil nucleic acid, with similar chemical characteristics, and therefore are difficult to separate.

Our Kit

A Mini kit to process up to 500 mg of soil samples
A Midi kit to process up to 2.5 g of soil samples
Bead tube with 3 different bead types in 5 different sizes
Total nucleic acid precipitation in 2 minutes
Patent pending pellet purification
Direct Precipitation
Silica membrane binding
Magnetic Beads based purification
Reduced hands-on time

Lyse’N’Trap           SLDMN-02 Series
Isolation and purification with the aid of silica membranes.
No separate purification steps for inhibitor removal
Bind lysate directly to spin columns
Wash and Elute
Complete the prep in less than 20 minutes

Lyse’N’Trap
Isolation and purification of nucleic acids with the aid of silica membranes.

Protocol Overview

Homogenization
Centrifugation
Binding
Washing
Elution

Mini Prep  

Protocol
Catalog Number Size Price
SLDMN-02-10 10 Preps $ 45.00
SLDMN-02-50 50 Preps $ 150.00
SLDMN-02-100 100 Preps $ 300.00

Midi Prep  

Protocol
Catalog Number Size Price
SLDMD-01-06 6 Preps $ 45.00
SLDMD-01-20 20 Preps $ 150.00

Direct Precipitation
Direct precipitation and purification of nucleic acids without the aid of solid phase purification.  Use same set of reagents to isolate and purify nucleic acids without the aid of silica membranes; great option to customize your protocol.  Suitable for soils with lower organic content.

Protocol

Magnetic Beads
Isolation and purification of nucleic acids with the aid of silica-coated magnetic beads with the same set of reagents.  Substitute magnetic beads instead of silica membrane spin columns.  A great way to scale-up your protocol and ideal for automated DNA isolations.

Protocol
Magnetic Beads
Reagents

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