Soil RNA Isolation

Our Kit

A Mini kit to process up to 500 mg of soil samples
A Midi kit to process up to 2.5 g of soil samples
Bead tubes with 3 different bead types in 5 different sizes
Total nucleic acid precipitation in 1 minute
Patent pending pellet purification
No Phenol:Chloroform purification
No Anion Exchange column purification
Silica membrane binding purification
Reduced hands-on time

The primary endeavor in microbial ecology is to define and understand the linkage between microbial community structures and their functions. The primary value of microbes is in their functions. Understanding the composition, relative abundance, biochemical diversity and dynamics of a microbial community will help us understand and eventually predict and manage functions in microbial communities of importance.
RNA-based community analysis is more suitable to describe the metabolically active members of a population, as the amount of rRNA produced by cells roughly correlates with the growth activity of bacteria.
The ubiquitous nature of RNases present in soil samples is probably the most important factor influencing the success of any protocol.  The other set of challenges is with humic acids and phenolic compounds that are major contaminants of soil RNA.

Lyse’N’Pellet
Direct precipitation and purification of RNA without the aid of solid phase purification.

Protocol Overview

Homogenization
                  ⇓
Centrifugation
                  ⇓
1 minute Precipitation
                  ⇓
Pellet Washing
                  ⇓
Precipitation
                  ⇓
Resuspension

Mini Prep            [maxbutton id=”3″]

  Catalog Number Size Price
SLRMN-01-10 10 Preps $ 45.00
SLRMN-01-50 50 Preps $ 150.00
SLRMN-01-100 100 Preps $ 275.00

Midi Prep            [maxbutton id=”3″]

  Catalog Number Size Price
SLRMD-01-10 10 Preps $ 45.00
SLRMD-01-50 20 Preps $ 120.00

 

Protocol

Lyse’N’Trap
Purification of RNA with the aid of silica membrane spin columns.

Protocol Overview

Homogenization
                 ⇓
Centrifugation
                ⇓
1 minute Precipitation
                ⇓
Pellet Washing
                ⇓
Binding
                ⇓
Washing
                ⇓
Elution

Mini Prep                    [maxbutton id=”3″]

  Catalog Number Size Price
SLRMN-02-10 10 Preps $ 45.00
SLRMN-02-50 50 Preps $ 200.00
SLRMN-02-100 100 Preps $ 350.00

Midi Prep             [maxbutton id=”3″]

  Catalog Number Size Price
SLRMD-02-10 10 Preps $ 45.00
SLRMD-02-50 20 Preps $ 170.00

 

[maxbutton id=”1″ text=”Homogenization” ]        [maxbutton id=”1″ text=”Spin Columns” ]        [maxbutton id=”1″ text=”Taq Enzymes” ]