DNA and RNA isolation from soil samples is limited in many ways becuase of co-extraction of organic contaminants other than nucleic acids. Humic acid and humic-like substances are the main group of contaminants that limit the downstream applications of nucleic acids. Nucleic and humic acids have similar characteristics, and are therefor difficult to separate. The other issue is isolation efficiency and because of the heterogenous nature of soil samples and the various organic contaminants, a portion of nucleic acid is invariably lost in the process of isolation. DNA and RNA extraction from soils is often more difficult than from many other ecological samples. It has been estimated that one gram of soil contains approximately 109 prokaryotes and more than 2000 genome types, with an average genome type representing less than 0.5% of the soil community. There is sufficient DNA in 1 g of soil to extend 993 miles.
Two sample Sizes Use our Mini Prep to porcess up to 500 mg of soil samples or our Midi Prep to process between 2 and 4 g of soils to obtain a better community profile and better yield with low biomass soil samples. Homogenization The homogenization efficiecny is significantly improved by using high density beads. Our specialized beads have a density of 6.2 g/cc while steel has a density of 7.9 g/cc, zirconia has a density of 5.5 g/cc and garnet has a density of 2.24 g/cc. We have five different sizes of beads in defined proportions to deal with differences in cell sizes. Compatible with a variety of homogenization tools.
Lysis Buffer The lysis buffer does it all. It preserves your samples after collection for up to 7 days at room temperature, resuspends and lyse the cells in combination with mechanical bead beating, denatures proteins and neutralizes humic acids and related compounds, preventing them from forming covalent bonds with nucleic acids.
Four Options for Purification To suit the needs of researchers, DNA can be isolated through three different techniques:
(i) Direct Precipitation without the aid of solid phase extraction. (ii) A simple and easy protocol using silica membranes.
(iii) Our magnetic beads-based protocol is readily amenable for automation.
(iv) Isolate sheared DNA for Next Generation Sequencing without the need for expensive instrumentations.