At BPI Tech, we are committed to revolutionize the art and science of sample preparation for biopolymer isolations and purifications, especially nucleic acids and proteins from a variety of biological sources. Our kits and isolation protocols are designed to meet the current requirements for Next generation high throughput sequencing of different biological samples. Our technology works around the type of biological samples processed, rather than blindly adopting a particular protocol to any and all biological samples. We place great emphasize on understanding the chemistry of the sample type, whether it is soil, plant, mammalian tissues, blood, fecal samples or microbes, and our isolation protocols work around the chemical composition of these types of samples. To keep up with the advances in instrumentation, we constantly strive to improve the sensitivity of our isolation protocols, placing importance on both, quantitative isolation and qualitative isolation. Our isolation methods include direct precipitation of nucleic acids from homogenized samples, use of silica membranes and silica-coated magnetic beads.
Nucleic Acid Isolation-Re visited
Nucleic Acid Isolation
Our nucleic acid extraction kits are quick, from homogenization to elution within 15 minutes, for any of your sample type. You have the option of hand homogenization or mechanical homogenization for plant and mammalian tissues. Irrespective of sample type, our protocols consist of five reagents: Lysis Buffer, Bind Buffer, Wash Buffer I, Wash Buffer II, and Elution Buffer. Wash Buffer II is 70% ethanol and Elution Buffer is sterile water. The core chemistry of our protocol is a patent-pending wash buffer, where polyphenolic contaminants are irrreversibly bound to silica membranes, preventing them from eluting with nucleic acids. This wash buffer also makes it possible to precipitate nucleic acids directly from lysates, and washing the pellets selectively resuspends nucleic acids without co-precipitated contaminants. This empowers end-users to customize protocols based on samples types and isolation needs. Through our field isolation kits, we enable researchers to process their samples immediately after collection, circumventing the need for storage and avoiding possible changes in biological profile of collected samples.
We offer a wide variety of lab accessories tailored to every step in your sample preparation. These include from homogenizing beads to Taq enzyme, and every thing in between.
We have a wide range of homogenizing beads to cover all your homogenizing needs for any sample type.
We have several options for silica membrane spin columns. The loading volumes range from 650 microliters to 60 ml. Our collection includes 6 different loading volumes and with an option to use in the field.
Our reagents include Lysis buffers for DNA and RNA isolation, along with corresponding Bind Buffers. We also offer clean-up reagent set to improve nucleic acid quality. This is a great way to customize our nucleic acid isolation for unique and challenging sample types.
Sample concentrator is a unique device that lets you concentrate nucleic acids from larger volumes. You can load up to 4.5 ml and concentrate onto a mini silica membrane spin columns. You can use a centrifuge or vacuum to concentrate your samples, and can do multiple loads of your samples.
The other accessories include nucleic acid storage cards, 96 well plates, magnetic beads and filtration columns with various pore sizes.