Nucleic Acid Concentrator

Multiples of 4.5 ml volume loading capacity

Concentrate nucleic acids from dilute solutions

Elute with your volume of choice; as low as 30 µl
Higher binding capacity membranes

Use with a centrifuge or vacuum manifold
In-built filters improves nucleic acid quality

Bulk pricing available  

Catalog Number        Quantity              Price       
 SC-01-50                        5                $ 80.00

 SC-01-100                      100                $ 160.00

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Protocol Overview 

Load samples up to 4.5 ml in the Midi filter cup   

Insert Concentrator Assembly in to a 15 ml tube 

 

Centrifuge at 5000 x g for 5 minutes

 

Discard flow-through in 15 ml tube 

⇓ 

Load another volume, if necessary; centrifuge as above   

Remove the mini spin columns (pull it down while holding the Midi column) 

 

Place the mini column in a 2 ml collection tube  

⇓ 

Centrifuge at 10000 x g for 1 minute to remove residual liquid trapped in silica membranes 

⇓ 

Wash mini columns with 500 µl of Wash Buffer (Centrifuge at 10,000 x g for 1 minute)   

Centrifuge empty mini columns one more time (Centrifuge at 10,000 x g for 1 minute) 

⇓ 

Transfer empty mini columns to a 1.5 ml collection tube   

 

Elute with appropriate volume of Elution Buffer (as low as 30 µl)

 (Centrifuge at 10,000 x g for 1 minute)