Nucleic Acid Concentrator
Multiples of 4.5 ml volume loading capacity
Concentrate nucleic acids from dilute solutions
Elute with your volume of choice; as low as 30 µl
Higher binding capacity membranes
Use with a centrifuge or vacuum manifold
In-built filters improves nucleic acid quality
Bulk pricing available
Catalog Number Quantity Price
SC-01-50 50 $ 80.00
SC-01-100 100 $ 160.00
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Protocol Overview
Load samples up to 4.5 ml in the Midi filter cup
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Insert Concentrator Assembly in to a 15 ml tube
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Centrifuge at 5000 x g for 5 minutes
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Discard flow-through in 15 ml tube
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Load another volume, if necessary; centrifuge as above
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Remove the mini spin columns (pull it down while holding the Midi column)
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Place the mini column in a 2 ml collection tube
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Centrifuge at 10000 x g for 1 minute to remove residual liquid trapped in silica membranes
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Wash mini columns with 500 µl of Wash Buffer (Centrifuge at 10,000 x g for 1 minute)
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Centrifuge empty mini columns one more time (Centrifuge at 10,000 x g for 1 minute)
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Transfer empty mini columns to a 1.5 ml collection tube
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Elute with appropriate volume of Elution Buffer (as low as 30 µl)
(Centrifuge at 10,000 x g for 1 minute)